Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods

Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods Michael Stevens, Jeffrey B. Cheng, Daofeng Li, Mingchao Xie, Chibo Hong, Cécile L. Maire, Keith L. Ligon, Martin Hirst, Marco A. Marra, Joseph F. Costello, and Ting Wang Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, Missouri 63108, USA; Department of Computer Science and Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA; Department of Dermatology, University of California, San Francisco, San Francisco, California 94143, USA; Brain Tumor Research Center, Department of Neurosurgery, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, California 94143, USA; Department of Medical Oncology, Center for Molecular Oncologic Pathology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215, USA; Departments of Pathology, Brigham and Women’s Hospital, Boston Children’s Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA; BC Cancer Agency, Canada’s Michael Smith Genome Sciences Centre Vancouver, British Columbia, V5Z 4S6, Canada; Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada